Sprühapplikation von respiratorischen Epithelzellen für das Tissue Engineering
- Spray application of respiratory epithelial cells for tissue engineering approaches
Thiebes, Anja Lena; Jockenhövel, Stefan (Thesis advisor); Dreher, Michael (Thesis advisor)
Dissertation / PhD Thesis
Dissertation, Rheinisch-Westfälische Technische Hochschule Aachen, 2016, Kumulative Dissertation
In the present dissertation, I examined the spray application of respiratory epithelial cells for tissue engineering purposes. In the first study (Thiebes et al., 2015) the general feasibility of spraying ovine epithelial cells is tested. It can be shown that air pressure of up to 0.8 bar and the cell suspension velocity do not impact survival of vascular smooth muscle cells. Respiratory epithelial cells have a significantly reduced viability of about 89 %. Still, it was successfully proven that the cells fully differentiate in vitro after spraying and build up cilia. Same applies for cells sprayed in fibrin gel, which helps immobilizing the cells at the desired position.Based on these good results, in the second study (Thiebes et al., 2016) we developed a new set-up for coating not only flat surfaces but also the inside of hollow organs or tubes, as stents, vascular grafts or airways with cells. The novel method is based on a conventional endoscope such that cell application onto the lumen can take place under direct vision. The focus here lies also on respiratory epithelium with regard to coating tracheal stents or prostheses with functional epithelium. It is shown that this spraying process does not influence the survival rate of epithelial cells. Additionally, the cells build a pseudostratified epithelium with cilia and goblet cells in vitro and are not impaired by the spraying process.The herein developed endoscopic cell application method is of high importance not only for tissue engineering purposes but can also make a major contribution to the field of cell therapy: Cells can be applied in fibrin locally at the organ of interest and can have an effect on-site and thereby increase the efficiency of the therapy.