Testung des Biofilminhibitors Carolacton im Kariesmodell der Ratte

Heinz, Vera Ute; Apel, Christian (Thesis advisor); Conrads, Georg (Thesis advisor)

Aachen (2015)
Dissertation / PhD Thesis


The dissertation describes the first application of carolacton in vivo. Carolacton is a biofilm inhibiting substance from Myxobacteria, which has already shown its inhibitory activity in nanomolar concentration in vitro. First we established a caries animal model for testing carolacton. After disinfection and antibiotic treatment, the animals (type Sprague Dawley) were inoculated with Streptococcus mutans, which is well known for playing a major role in the caries development. The in vivo activity of carolacton was tested in two animal trials. In the first experiment it was used as dental varnish and in the second experiment it was used as drinking water additive and mouthwash. In the second experiment fluoride served as positive control for any cariogenic effect. In dentistry fluoride is traditionally used for caries prophylaxis. At the end of the experimental series (duration 67 and 41 days) the molar teeth of the animals and the caries activity were evaluated histological. The evaluation was carried out by methods according to Keyes, Larson and the new developed “Heinz”-scoring method. Statistical data were derived from the histological test result and analyzed by the use of a statistical significance test (ANOVA). With the help of carolacton a reduction of the caries activity should have been achieved. The caries induction was successful in both test-runs. In the first test run by Keyes method a significant difference can be detected between the control-group and the varnish group C (p=0,0069) and the control-group and the varnish group D (without carolacton), too (p=0,0266). By analysis of the single values (E, Ds, Dm, Dx) this significance can be found in the lower dentin layers (Ds, Dm, Dx). No significance can be found in the dental enamel area (E) of all groups (p=0,8723). In the second test run (according to Keyes) a significant difference between the fluoride-group and the carolacton-B-group can be seen (p=0,0245). The analysis of the single values shows a significant difference in the Ds-area (p=0,0005). Another significance can be found between the fluoride-group and the carolacton-A-group (without carolacton) in the Ds-area (p=0,0326). The use of carolacton could not achieve any biofilm inhibiting results in these experiments.